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Objective:To investigate the serum level of fatty acid binding protein 1 (FABP1) and its relationship with Helicobacter pylori (Hp) infection in patients with gastric cancer. Methods:Forty gastric cancer patients (gastric cancer group) who were hospitalized in Affiliated Hospital of Qinghai University from August 2021 to August 2022 were selected as the research subjects, and 40 physical examination subjects during the same period were selected as the normal control group and 40 chronic atrophic gastritis patients were selected as the CAG group. The Hp infection were detected by 13C breath test, and the levels of serum FABP1 were detected by enzyme-linked immunosorbent assay. The Hp infection status, serum FABP1 levels, and the relationship between the two in the three groups of study subjects were analyzed. And the relationships between the level of serum FABP1 and the clinicopathological features of gastric cancer patients were analyzed. The diagnostic value of serum FABP1, CA19-9, CA72-4 and combined test of 3 indexes were evaluated by receiver operating characteristic (ROC) curve. Results:The Hp infection rates in the control group, CAG group, and gastric cancer group were 32.50% (13/40), 55.00% (22/40), and 60.00% (24/40), respectively, with a statistically significant difference ( χ2=6.87, P=0.032). Among them, the Hp infection rate in the control group was compared with that in the gastric cancer group, with a statistically significant difference ( P<0.05), and there were no statistically significant differences between the CAG group and the control group, the gastric cancer group (both P>0.05). The levels of serum FABP1 in the control group, CAG group, and gastric cancer group were [63.47 (37.53, 71.59) ] ng/ml, [65.26 (51.15, 79.67) ] ng/ml, and [72.84 (53.44, 82.25) ] ng/ml, respectively, with a statistically significant difference ( H=6.62, P=0.037). Among them, there was a statistically significant difference between the control group and the gastric cancer group ( H=19.93, P=0.031), while there were no statistically significant differences between the CAG group and the control group, the gastric cancer group ( H=1.50, P=0.133; H=1.09, P=0.277). Among all study subjects, the levels of serum FABP1 in the Hp positive group ( n=59) and Hp negative group ( n=61) were [77.05 (68.90, 83.54) ] ng/ml and [47.80 (37.76, 63.32) ] ng/ml, respectively, with a statistically significant difference ( Z=7.45, P<0.001). In the control group, the levels of FABP1 in the serum of Hp positive and Hp negative persons were [77.34 (68.84, 86.31) ] ng/ml and [39.79 (36.83, 63.75) ] ng/ml, respectively, with a statistically significant difference ( Z=4.46, P<0.001). In the CAG group, the levels of FABP1 in the serum of Hp positive and Hp negative patients were [76.51 (65.30, 80.97) ] ng/ml and [49.34 (39.92, 59.41) ] ng/ml, respectively, with a statistically significant difference ( Z=4.32, P<0.001). In the gastric cancer group, the levels of FABP1 in the serum of Hp positive and Hp negative patients were [77.15 (72.62, 84.13) ] ng/ml and [50.57 (44.54, 68.97) ] ng/ml, respectively, with a statistically significant difference ( Z=4.32, P<0.001). There were significant correlations between the serum level of FABP1 and smoking ( t=2.54, P=0.015), tumor diameter ( t=2.23, P=0.035), and lymph node metastasis ( t=3.22, P=0.003) in gastric cancer patients. And there were no significant correlations between FABP1 and gender ( t=0.98, P=0.333), age ( t=1.60, P=0.117), alcohol consumption ( Z=0.10, P=0.925), tumor site ( F=1.06, P=0.356), degree of differentiation ( t=0.61, P=0.545), the depth of infiltration ( t=1.41, P=0.166), distant metastasis ( Z=1.96, P=0.050) and TNM staging ( Z=0.66, P=0.508). ROC curve analysis showed that the area under the curve (AUC) of serum FABP1 for gastric cancer diagnosis was 0.62, 95% CI: 0.51-0.72, the sensitivity and specificity were 57.50% and 68.70%, respectively; the AUC of CA19-9 for gastric cancer diagnosis was 0.89, 95% CI: 0.83-0.95, the sensitivity and specificity were 77.50%, 86.30%, respectively; the AUC of CA72-4 for gastric cancer diagnosis was 0.88, 95% CI: 0.81-0.94, the sensitivity and specificity were 70.00%, 93.70%, respectively; the AUC of combined test of 3 indexes for gastric cancer diagnosis was 0.91, 95% CI: 0.82-0.97, the sensitivity and specificity were 67.50% and 95.00%, respectively. Conclusion:The Hp infection rate of gastric cancer patients is higher than that of the health examiners, the serum FABP1 level of gastric cancer patients is higher than that of the healthy health examiners, the serum FABP1 level of Hp positive persons is higher than that of Hp negative persons, and Hp infection and FABP1 level may have a common carcinogenic mechanism in the occurrence and development of gastric cancer.
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Abstract Background: Vitiligo is an acquired and progressive mucocutaneous disease resulting from the loss of active epidermal melanocytes. Metabolic syndrome (MetS) affects about 25% of the world's population and is linked to inflammatory skin diseases including vitiligo. Fatty AcidBinding Protein 4 (FABP4) is an intracellular lipid chaperone. FABP4 is closely associated with MetS. Objectives: To evaluate the serum level of FABP4 in vitiligo patients and its relation to MetS in the investigated cases. Methods: This case control study was conducted on 45 patients having non segmental vitiligo and 45 matched controls. Their lipid profile, blood glucose and serum FABP4 levels were measured. Results: There were significant elevations in FABP4 (p < 0.001), cholesterol (p < 0.001), triglycerides (p = 0.005), and glucose (fasting [p = 0.001] and 2 hours post prandial [p < 0.001]) levels in patients in comparison with controls. MetS was significantly more prevalent among vitiligo patients (p < 0.001) and associated with high FABP4 serum levels (p = 0.037). In vitiligo patients, there were significant positive correlations between FABP4 serum levels and triglycerides (p = 0.047), cholesterol (p = 0.001) and LDL (p = 0.001) levels and negative correlation regarding HDL level (p = 0.009). FABP4 level was a significantly good diagnostic test for early detection of vitiligo (p < 0.001). Study limitations: The small number of studied subjects. Conclusions: FABP4 may play an active role in the disease process of vitiligo that could be mediated through associated dyslipidemia and hyperglycemia. FABP4 may be a marker of vitiligo helping in its early diagnosis, but it does not appear to be useful for determining vitiligo severity, activity or associated MetS.
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Humans , Metabolic Syndrome , Fatty Acid-Binding Proteins/blood , Triglycerides , Vitiligo , Case-Control StudiesABSTRACT
Objective@#To investigate serum level changes of heart-type fatty acid-binding protein(H-FABP)and S100 calcium-binding protein B(S-100B)protein in elderly patients with chronic heart failure and their clinical significance.@*Methods@#A total of 160 patients with chronic heart failure treated at our hospital were recruited, and 80 healthy individuals receiving regular check-ups were enrolled as normal controls.Serum levels of H-FABP and S-100B and cardiac function index scores were compared between patients with different cardiac function grades.Correlations of serum H-FABP and S-100B levels with N-terminal pro-B-type natrlure tiepeptide(NT-proBNP)and with cardiac function index scores in heart failure patients were analyzed.The sensitivity, specificity and accuracy of serum H-FABP, S-100B and NT-proBNP for heart failure detection were compared.@*Results@#Serum levels of H-FABP, S-100B and NT-proBNP in elderly patients with chronic heart failure were elevated with increased cardiac function grading(F=9.823, 11.573 and 13.056, P=0.013, 0.000 and 0.000), and serum levels of H-FABP, S-100B and NT-proBNP were higher in elderly patients with heart failure than in the control group(P<0.05). Serum levels of H-FABP and S-100B were positively correlated with serum NT-proBNP levels, cardiac function grading and left ventricular end-diastolic diameter(LVEDd)(r=0.527, 0.510 and 0.487, P=0.008, 0.003 and 0.002; r=0.604, 0.496 and 0.533, P=0.006, 0.005 and 0.003), and were negatively correlated with left ventricular ejection fraction(LVEF)(r=-0.536 and-0.528, P=0.005 and 0.008). The sensitivity, specificity and accuracy of serum H-FABP combined with S-100B for heart failure detection were 93.2%, 91.6% and 95.7%, respectively.@*Conclusions@#Serum levels of H-FABP and S-100B are high in elderly patients with heart failure, and they are correlated with serum NT-proBNP levels, cardiac function grading and LVEDd.H-FABP combined with S-100B has a high positive rate for heart failure detection.
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Objective To investigate serum level changes of heart-type fatty acid-binding protein(H-FABP)and S100 calcium-binding protein B(S-100B)protein in elderly patients with chronic heart failure and their clinical significance.Methods A total of 160 patients with chronic heart failure treated at our hospital were recruited,and 80 healthy individuals receiving regular check-ups were enrolled as normal controls.Serum levels of H-FABP and S-100B and cardiac function index scores were compared between patients with different cardiac function grades.Correlations of serum H-FABP and S-100B levels with N-terminal pro-B-type natrlure tiepeptide(NT-proBNP)and with cardiac function index scores in heart failure patients were analyzed.The sensitivity,specificity and accuracy of serum H-FABP,S-100B and NT-proBNP for heart failure detection were compared.Results Serum levels of H-FABP,S-100B and NT-proBNP in elderly patients with chronic heart failure were elevated with increased cardiac function grading (F =9.823,11.573 and 13.056,P =0.013,0.000 and 0.000),and serum levels of H-FABP,S-100B and NT-proBNP were higher in elderly patients with heart failure than in the control group(P＜0.05).Serum levels of H-FABP and S-100B were positively correlated with serum NT-proBNP levels,cardiac function grading and left ventricular end-diastolic diameter(LVEDd) (r =0.527,0.510 and 0.487,P =0.008,0.003 and 0.002;r =0.604,0.496 and 0.533,P =0.006,0.005 and 0.003),and were negatively correlated with left ventricular ejection fraction(LVEF) (r =-0.536 and-0.528,P =0.005 and 0.008).The sensitivity,specificity and accuracy of serum H-FABP combined with S-100B for heart failure detection were 93.2％,91.6％ and 95.7％,respectively.Conclusions Serum levels of H-FABP and S-100B are high in elderly patients with heart failure,and they are correlated with serum NT-proBNP levels,cardiac function grading and LVEDd.H-FABP combined with S-100B has a high positive rate for heart failure detection.
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To analyze the relationship among serum heart type fatty acid binding protein (HFABP) , ho‐mocysteine (Hcy) levels and ventricular remodeling , prognosis in patients with chronic heart fail (CHF).Methods :A total of 128 patients with CHF admitted to our hospital from May 2013 to Nov 2015 were enrolled .According to the New York Heart Association (NYHA) cardiac function grading , patients were divided into three groups : grade II (n＝48) , grade III (n＝42) and grade IV (n＝38) , at the sane time 35 patients with heart function grade I as a control group .Cardiac examination was performed by echocardiography to obtain parameters related to left ventric‐ular remodeling .The levels fo serum H‐FABP and Hcy were measured on the next day of admission , and correlation analysis was performed .All patients were treated with targeted cardiac therapy and followed up for 24 months.The cardiac events were used as the end point of the study , indicating poor prognosis .The relationship among serum H‐FABP and Hcy levels and prognosis was analyzed .Results : Left ventricular end diastolic diameter (LVEDd ) [ (40.40 ± 4.41) mm vs .(42. 64 ± 3.22) mm vs.(45.20 ± 3. 41 ) mm vs.(46. 94 ± 4.22) mm] , left ventricular posterior wall thickness (LVPW) [ (8.45 ± 1.15) mm vs.(9. 04 ± 1. 21) mm vs.(10.05 ± 1.35) mm vs.(11. 94 ± 1.27) mm] , and interventricular septum thickness (IVS) [ (7. 01 ± 0.92) mm vs.(8.93 ± 1. 12) mm vs.(10. 09 ± 1.29) mm vs.(11. 93 ± 1. 32) mm] were significantly increased in patients with grade I , II , III , and IV , and left ventricular ejection fraction ( LVEF) [ (52.16 ± 4.02)% vs.(50. 32 ± 3.29)% vs .(48.16 ± 3. 32)% vs.(45.32 ± 2.29)%] decreased significantly ( P＜0. 05 or P＜0. 01) , and serum H‐FABP [ (3. 36 ± 0.71 ) ng／L vs.(4. 13 ± 1.13) ng／L vs .(5. 65 ± 1.42) ng／L vs.(6.49 ± 1. 69) ng／L] and Hcy [ (9. 46 ± 1.55) μmol／L vs.(14. 49 ± 2. 60) μmol／L vs.(17.71 ± 3. 61) μmol／L vs.(20. 26 ± 3. 37 ) μmol／L ] levels increased significantly ( P＜ 0.05 or P＜0.01).Pearson correlation analysis showed that serum H‐FABP and Hcy levels were positively correlated with LVEDd , IVS and LVPW ( r＝0.312～0. 392 , P＜0.05) , and negatively correlated with LVEF ( r＝ －0.453 ,－0.484 , P＜0.05).During 24 months of follow‐up , 56 patients (43. 75%) with CHF had a poor prognosis , com‐pared with the prognosis group , serum H‐FABPP [ (4. 74 ± 1.43) ng／L vs.(6.27 ± 1.28 ) ng／L ] and Hcy level [ (11.35 ± 2.03) μmol／L vs.(18.33 ± 3.46) μmol／L] in the poor prognosis group were significantly increased (P＝0.001 ).Conclusion : There is certain correlation between serum H‐FABP and Hcy levels and ventricular remodeling in patients with CHF , which plays an important role in the assessment of disease and prognosis .
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Objective To study the expression of intestinal intestinal fatty acid-binding protein (I-FABP) and its clinical significance in an experimental model of necrotizing enterocolitis (NEC) in neonatal rats. Method Twenty-four neonatal Sprague-Dawley (SD) rats were randomly assigned into 4 groups at 48 h after birth (6 rats in each group):group A (control group), group B (NEC group-1), group C (NEC group-2), and group D (NEC group-3). The neonatal rats were fed by the mother rats in the same cage within 48 h after birth. After 48 h, the NEC group received artificial feeding, hypoxia, cold stimulation and lipopolysaccharide (LPS) gavage (10 mg/kg). NEC group-1, 2 and 3 were sacrificed on an empty stomach at 1, 2 and 3 d after the modeling. The control group was sacrificed on an empty stomach 3 d after the modeling without special treatment. Intestinal tissue were obtained from each rats. The histological changes of ileal tissues were studied using hematoxylin-eosin (HE) staining. The expressions of intestinal I-FABP were detected using RT-PCR and ELISA methods. Result Compared with the control group, body weight of rats in NEC group-1, 2 and 3 were lower, and pathology scores in these three groups were higher (P<0.05). The levels of intestinal I-FABP mRNA in NEC group-1, NEC group-2 and NEC group-3 were 2.69±0.27, 2.12±0.09, 3.18± 0.22, respectively. The protein expression levels were 363.7 ± 11.4, 321.7 ± 45.8, 432.3 ± 50.3, respectively. The mRNA and protein levels were all significantly higher than the control group (mRNA: 1.00 ± 0.02, protein: 134.2 ± 24.0, P<0.05). Conclusion I-FABP was a useful marker for ischemic injury to the intestine. These findings may contribute to a better diagnosis of NEC in newborns.
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Objective To analyze the role of intestinal fatty acid-binding protein (I-FABP) expression in a neonatal rat model of necrotizing enterocolitis (NEC).Methods A total of 24 newborn rats were randomly divided into two groups:control group (n=6) and NEC group (n=18).Rats in the NEC group were fed with formula and experienced hypoxia,reoxygenation,cold stress and sequentially Lipopolysaccharide (10 mg/kg) lavage for three consecutive days to establish NEC model,after which were respectively sacrificed on day 1,2 and 3 (six for each day).Those in the control group were all sacrificed on day 3.Ileocecal tissues were collected for morphological and histological analysis.I-FABP expression was detected using Western blot and immunohistochemistry (IHC).One-way analysis of variance,LSD-t test,Kruskal-Wallis H test,Mann-Whitney U test and Pearson's correlation analysis were used for statistical analysis.Results The NEC model (intestinal pathological score ≥ 2) was established successfully without causing death.Compared with the control group,the NEC group showed less body weight gain [M (P25-P75):1.00 (0.48-1.35) vs 1.74 (1.62-1.86),1.25 (0.75-1.40) vs 2.61 (2.53-2.99),1.35 (0.88-1.48) vs 3.60 (3.48-3.73);Z=-2.898,-2.903,-2.892;all P＜0.05] and higher intestinal pathological scores [(2 (2-3),3 (2-3),4 (3-4) vs 0 (0-1);all P＜0.05] on day 1,2 and 3.The intestinal pathological score on day 3 was significantly higher than that on day 2 and day 1 (both P＜0.05).Expression of I-FABP and the number of I-FABP positive enterocytes in the NEC model group were increased compared with those in the control group [Western blot:0.179 (0.179-0.186),0.231 (0.211-0.245),0.202 (0.192-0.225) vs 0.091 (0.086-0.093);IHC:59 (55-60),80 (83-86),80 (84-88) vs 44 (39-47);all P＜0.05].Moreover,the expression of I-FABP protein and the number of I-FABP positive enterocytes on day 2 and day 3 were significantly higher than those on day 1 (all P＜0.05).I-FABP expression was positively associated with intestinal pathological score (Western blot:r=0.932,95％CI:0.872-0.969;IHC:r=0.709,95％CI:0.484-0.872).Conclusions I-FABP is an efficient marker for NEC and correlates with the severity of intestinal injury.
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Objective To examine the practical value of early detection of heart-type fatty acid binding protein (H-FABP)for risk stratification and prognosis assessment in cardiac troponin T (cTnT)-negative acute coronary syndrome(ACS)patients.Methods From March 2010 to March 2012,55 patients with chest pain and negative cTnT were selected from 232 ACS patients at the General Hospital of PLA.Expression levels of cTnT and H-FABP were detected within 6 h of the onset of clinical symptoms.H-FABP and cTnT values at 12,24,and 48 h from the onset of clinical symptoms were continuously measured to monitor the dynamic changes.Based on prognosis,patients were divided into two groups,levels of H-FABP were compared,and its predictive value for prognosis was assessed with the ROC curve.Results Within 6 h of the onset of clinical symptoms,cTnT levels in cTnT-negative ACS patients increased gradually as disease progressed and reached the peak value at 12 h before decreasing slowly and arriving at 50％ of the peak value at 48 h.Meanwhile,HFABP levels reached the peak within 6 h,decreased slightly(12.8％) at 12 h,and then decreased rapidly at 48 h (about 79％).Of 55 patients,24 had acute myocardial infarction during hospitalization.The H-FABP level within 6 h was a good predictor for cTnT-negative ACS patients.The area under ROC curve was 0.946 and the cutoff value was 15.47 μg/L.The prediction sensitivity was 87.5 ％,with a specificity of 90.3％.Eleven patients had cardiovascular events after a 12-month follow-up.Levels of H-FABP were different in patients with or without cardiovascular events,[(38.08±8.43) μg/L vs.(18.96 ± 2.85) μg/L (t =2.438,P＜0.05)].ROC curve analysis showed that the area under the curve was 0.772 and the prediction cutoff value was 44.71 μg/L.The rates of cardiovascular events were markedly different between patients with high(≥44.71 μg/L)and those with 1ow(＜44.71 μg/L)H-FABP levels(54.5％ vs.11.4％).Conclusions For ACS patients with negative cTnT,H-FABP is a good index for early risk stratification and prognosis assessment.
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Objective:To study diagnostic value of combined detection of heart type fatty acid binding protein (H-FABP) and brain natriuretic peptide (BNP) for chronic heart failure (CHF) in aged patients.Methods:A total of 178 aged CHF patients hospitalized in our hospital from Jul 2015 to Jun 2016 were selected.According to NYHA cardiac function classification,they were divided into class Ⅱ group (n＝70) and class Ⅲ ～ Ⅳ group (n＝48).An-other 52 aged healthy subjects undergoing physical examination were selected as healthy control group simultaneous-ly.Plasma BNP,H-FABP levels,LAD,LVESd,LVEDd,IVST and LVEF were measured and compared among three groups.Correlation among plasma BNP,H-FABP levels and above cardiac function indexes were analyzed.Results:Compared with healthy control group,there were significant rise in plasma levels of BNP [ (50.65 ± 5.86) ng/L vs.(212.65 ± 17.12) ng/L vs.(1264.73 ± 119.74) ng/L] and H-FABP [ (1.68 ± 0.36) ng/ml vs.(3.34 ± 0.56) ng/ml vs.(9.64 ± 1.27) ng/ml],LAD [ (3.25 ± 0.29) cm vs.(4.98 ± 0.23) cm vs.(5.48 ± 0.51) cm],LVESd [ (28.68 ± 2.7) mm vs.(40.98 ± 4.55) mm vs.(58.63 ± 5.75) mm],LVEDd [ (43.22 ± 4.29) mm vs.(56.63 ± 5.23) mm vs.(61.33 ± 6.07) mm] and IVST [ (8.15 ± 0.68) mm vs.(11.87 ± 1.03) mm vs.(15.93 ± 1.43) mm],and significant reduction in LVEF [ (64.84 ± 6.39)% vs.(49.76 ± 4.76)% vs.(35.76 ± 3.62)%] in NYHA class Ⅱ group and NYHA class Ⅲ ～ Ⅳ group;compared with NYHA class Ⅱ group,there were significant rise in plasma levels of BNP and H-FABP,LAD,LVESd,LVEDd and IVST,and significant reduction in LVEF in NYHA class Ⅲ ～ Ⅳ group,P＝0.001 all.Spearman correlation analysis indicated that plasma BNP,H-FABP levels were significant positively correlated with LAD,LVESd,LVEDd and IVST (r＝0.53～0.71,P＝0.001 all),and significant inversely correlated with LVEF (r＝ -0.54,-0.65,P＝0.001 both);plasma BNP level was signifi-cantly positive correlated with H-FABP level (r＝0.36,P＝0.04).Conclusion:Combined detection of BNP and H-FABP contributes to diagnosing CHF in aged patients and assessing its severity.
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Objective To investigate the effect of Yanhuning injection antiviral therapy in the treatment of acute viral myocarditis patients with Xiedu Qinxin syndrome.Methods From May 2016 to August 2017,84 acute viral myocarditis patients with Xiedu Qinxin syndrome in the Integrated Traditional Chinese and Western Medicine Hospital of Wenzhou were enrolled in this study.According to the digital table,they were randomly divided into observation group and the control group,with 42 cases in each group.The control group received conventional antiviral therapy,and the observation group was given Yanhuning injection.The heart type free fatty acid binding protein (H－FABP),serum troponin Ⅰ (cTnI),macrophage migration inhibitory factor(MIF)and interleukin 4(IL－4)were measured before and after treatment for 4 weeks.The clinical symptoms score and the effective rate were compared between the two groups at the same time.Results The serum levels of cTnI,H－FABP,MIF and IL－4 of the two groups after treatment were lower than those before treatment(all P＜0.05 ),which of the observation group after treatment were significantly lower than those of the control group (t＝3.012,P＝0.039;t＝2.835,P＝0.040;t＝3.534,P＝0.032;t＝3.323,P＝0.033).The effective rate of the observation group was significantly higher than that of the control group (90.48% vs.80.95%,χ2＝3.432,P＝0.038).The scores of palpitations,sore throat,upsetting the chest tightness of the observation group after treatment were significantly lower than those of the control group (t＝3.045,P＝0.038;t＝2.946,P＝0.039;t＝3.467,P＝0.031;t ＝3.358,P＝0.032).Conclusion Yanhuning injection antiviral therapy in the treatment of acute viral myocarditis patients with Xiedu Qinxin syndrome can signifi-cantly improve the efficacy of patients.
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Objective:To explore relationship among serum levels of adipocyte fatty acid binding protein (A-FABP), chemokine CXCL12 and acute ischemic stroke (AIS).Methods:A total of 92 aged AIS patients treated in our hospi-tal were selected as AIS group,another 83 healthy subjects undergoing physical examination in our hospital were en-rolled as healthy control group.Serum levels of A-FABP and chemokine CXCL12 were measured and compared be-tween two groups;United states national institutes of health stroke score (NIHSS)was used to assess neurological function deficit degree;Glasgow coma scale (GCS)was used to judge their consciousness.AIS patients were fol-lowed up for three months,then modified Rankin rating score (mRS)was used to assess their prognosis.Results:Compared with healthy control group,there were significant rise in serum levels of A-FABP [(20.92±2.63)μg/L vs.(35.63±5.72)μg/L]and chemokine CXCL12 [(4.25 ±0.61 )ng/ml vs.(24.31 ±3.46)ng/ml]in AIS group,P =0.001 both.Compared with small-medium size infraction group,NIHSS score≤15 scores group,GCS score≤8 scores group and good prognosis group,the levels of FABP [(32.89±5.34)μg/L,(31.72±4.15)μg/L, (30.68±4.21)μg/L,(32.17±3.72)μg/L vs.(38.24±5.86 )μg/L,(39.58±4.28)μg/L,(40.97±4.24)μg/L,(38.92±4.35)μg/L;chemokine CXCL12 [(21.38±3.18)ng /ml, (21.03 ±2.45)ng/ml,(20.78±2.42) ng/ml,(21.54±2.67)ng/ml vs.(28.26±3.37)ng/ml,(27.18±2.94)ng/ml,(27.51±2.89)ng/ml,(27.23±3.15)ng/ml]significantly rose (P <0.01 all)in large size infarction group,NIHSS score > 15 scores group,GCS score>8 scores group and poor prognosis group.Conclusion:Serum levels of A-FABP and chemokine CXCL12 are closely related to occurrence and severity of AIS,which can be used as important indexes for early diagnosis,disease monitor and prognosis assessment in AIS patients.
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Objective To investigate the relationship between insulin resistance and FABP4,FGF21 and Nesfatin-1 in patients with gestational diabetes mellitus ( GDM) . Methods 60 patients with GDM were selected as the observation group,60 healthy pregnant women served as control group. The indicators of blood lipid,blood sugar, insulin resistance and the levels of FABP4, FGF21 and Nesfatin -1 were compared between the two groups. The correlation of FABP4,FGF21,Nesfatin -1 and insulin resistance was analyzed. Results The FABP4 [(27. 39 ± 3.58)μg/Lvs.(18.41±3.62)μg/L],FGF21[(193.29±17.31)ng/Lvs.(102.83±18.33)ng/L],Nesfatin-1 [(2. 49 ± 0. 61)μg/L vs. (1. 97 ± 0. 56)μg/L] levels of the observation group were significantly higher than those of the control group,the differences were statistically significant (t =13. 662,27. 728,4. 862,all P <0. 05). Pearson correlation analysis showed that FABP4,FGF21,Nesfatin-1 (r=0. 489,0. 368,0. 968,all P<0. 05) were positively associated with HOMAIR,and were negatively correlated with HOMA beta,ISI ( r= -0. 567,-0. 389,-0. 722,-0. 497,-0. 674,-0. 957,all P<0. 05). Conclusion There is obvious disorder of glucose and lipid metabolism and insulin resistance in patients with GDM. The serum levels of FABP4,FGF21 and Nesfatin-1 are significantly increased,and are related to insulin resistance.
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Background:The prognosis of systemic lupus erythematosus complicated with lupus enteropathy is poor. At present, studies on pathogenesis of lupus enteropathy are rare. Aims:To investigate the expressions of intercellular adhesion molecule-1 (ICAM-1),vascular cell adhesion molecule-1 (VCAM-1)and intestinal fatty acid binding protein (I-FABP) in lupus enteropathy in mice. Methods:Twenty MRL/ lpr lupus mice were randomly divided into two groups:lupus enteropathy group and control group. Lupus enteropathy model was established by administration with TNBS enema. Histological score was assessed,expressions of ICAM-1,VCAM-1 and I-FABP were determined by immunohistochemistry, and correlations with histological score were analyzed. Results:Compared with control group,histological score was significantly increased (8. 1 ± 5. 8 vs. 0. 8 ± 0. 5,P = 0. 000),expressions of ICAM-1 (9. 4% ± 2. 1% vs. 6. 2% ± 1. 1%),VCAM-1 (15. 1% ± 2. 1% vs. 12. 2% ± 1. 9%)and I-FABP (17. 5% ± 2. 5% vs. 6. 1% ± 0. 9%)were significantly increased (P < 0. 05)in lupus enteropathy group. Expressions of ICAM-1,VCAM-1 and I-FABP in colon tissue in lupus enteropathy group were positively correlated with histological score (r = 0. 870,P = 0. 010;r = 0. 881,P =0. 010;r = 1. 000,P = 0. 000). Conclusions:ICAM-1,VCAM-1 and I-FABP may be associated with pathogenesis of lupus enteropathy.
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Objective To study the diagnostic value of serologic intestinal-fatty acid binding protein (i-FABP) in neonatal necrotizing enterocolitis (NEC).Method The standard search strategy included electronic search and manual search.Electronic search was carried out in databases including PubMed,Ovid,Cochrane Library,Chinese Biomedicine Database (CBM),CNKI,WANFANG DATA and VIP database.As a supplement,relevant references from previous reviews and studies were examined by manual search.Two independent reviewers assessed the quality of included studies and extracted data,and performed further meta-analysis.RevManS.3.5 and Meta-Disc1.4 and Stata12.0 were used in the statistical analysis.Result A total of 11 studies with 700 cases were identified,including 308 NEC cases and 392 control cases.The blood samples collected at the time of suspected NEC stage showed pooled sensitivity 0.56 (95％ CI 0.44 ～ 0.67),pooled specificity 0.93 (95 ％ CI0.87 ～ 0.97),area under curve (AUC) =0.8282,and Q* =0.7610,respectively.The blood samples collected after clinical diagnosis of NEC showed pooled sensitivity 0.78 (95％ CI 0.72 ～ 0.83),pooled specificity 0.81 (95％ CI 0.76 ～ 0.86),AUC =0.8680,and Q* =0.7985,respectively.Conclusion i-FABP is a valid serologic biomarker for early diagnosis of NEC in neonatal infants.
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Objective To investigate the value of urine liver?type fatty acid?binding proteins(L?FABP) for early diagnosis and progress predicting of acute kidney injury(AKI)after lung transplantation. Methods Urine L?FABP and Scr blood samples in perioperative periods of 119 lung transplant recipients (hospitalized between 2013?2014)were involved in the research. Patients were divided into AKI group and non?AKI group according to KDIGO. Changes in urine L?FABP and Scr of two groups at various time points were recorded. Results Of 119 patients,57 developed AKI after surgery. Urine L?FABP from 0 h to 48 h in the two groups increased significantly, and the difference at 6 h to 48 h between the two groups is significant. In terms of diagnostic value,ROC area of urine L?FABP at 6h is 0.818. When 2254.52 ng/mg Cr was taken as diagnostic dividing line ,sensitivity and specificity was 0.782 and 0.814. In predicting AKI progression ,AUC below AUC of urine L?FABP 0.852. When 4313.17 ng/mgCr was taken as diagnostic dividing line ,sensitivity and specificity was 0.867 and 0.700. Conclusion Urinary L?FABP appears to be a sensitive and specific marker of AKI in lung transplant recipients ,could be a biological marker in the early diagnosis and progression tendency of AKI.
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Objective:To explore relationship among serum levels of adipocyte fatty acid binding protein (A-FABP), chemokine CXCL12 and acute ischemic stroke (AIS).Methods:A total of 92 aged AIS patients treated in our hospi-tal were selected as AIS group,another 83 healthy subjects undergoing physical examination in our hospital were en-rolled as healthy control group.Serum levels of A-FABP and chemokine CXCL12 were measured and compared be-tween two groups;United states national institutes of health stroke score (NIHSS)was used to assess neurological function deficit degree;Glasgow coma scale (GCS)was used to judge their consciousness.AIS patients were fol-lowed up for three months,then modified Rankin rating score (mRS)was used to assess their prognosis.Results:Compared with healthy control group,there were significant rise in serum levels of A-FABP [(20.92±2.63)μg/L vs.(35.63±5.72)μg/L]and chemokine CXCL12 [(4.25 ±0.61 )ng/ml vs.(24.31 ±3.46)ng/ml]in AIS group,P =0.001 both.Compared with small-medium size infraction group,NIHSS score≤15 scores group,GCS score≤8 scores group and good prognosis group,the levels of FABP [(32.89±5.34)μg/L,(31.72±4.15)μg/L, (30.68±4.21)μg/L,(32.17±3.72)μg/L vs.(38.24±5.86 )μg/L,(39.58±4.28)μg/L,(40.97±4.24)μg/L,(38.92±4.35)μg/L;chemokine CXCL12 [(21.38±3.18)ng /ml, (21.03 ±2.45)ng/ml,(20.78±2.42) ng/ml,(21.54±2.67)ng/ml vs.(28.26±3.37)ng/ml,(27.18±2.94)ng/ml,(27.51±2.89)ng/ml,(27.23±3.15)ng/ml]significantly rose (P <0.01 all)in large size infarction group,NIHSS score > 15 scores group,GCS score>8 scores group and poor prognosis group.Conclusion:Serum levels of A-FABP and chemokine CXCL12 are closely related to occurrence and severity of AIS,which can be used as important indexes for early diagnosis,disease monitor and prognosis assessment in AIS patients.
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Objective To investigate the relationship between insulin resistance and FABP4,FGF21 and Nesfatin-1 in patients with gestational diabetes mellitus ( GDM) . Methods 60 patients with GDM were selected as the observation group,60 healthy pregnant women served as control group. The indicators of blood lipid,blood sugar, insulin resistance and the levels of FABP4, FGF21 and Nesfatin -1 were compared between the two groups. The correlation of FABP4,FGF21,Nesfatin -1 and insulin resistance was analyzed. Results The FABP4 [(27. 39 ± 3.58)μg/Lvs.(18.41±3.62)μg/L],FGF21[(193.29±17.31)ng/Lvs.(102.83±18.33)ng/L],Nesfatin-1 [(2. 49 ± 0. 61)μg/L vs. (1. 97 ± 0. 56)μg/L] levels of the observation group were significantly higher than those of the control group,the differences were statistically significant (t =13. 662,27. 728,4. 862,all P <0. 05). Pearson correlation analysis showed that FABP4,FGF21,Nesfatin-1 (r=0. 489,0. 368,0. 968,all P<0. 05) were positively associated with HOMAIR,and were negatively correlated with HOMA beta,ISI ( r= -0. 567,-0. 389,-0. 722,-0. 497,-0. 674,-0. 957,all P<0. 05). Conclusion There is obvious disorder of glucose and lipid metabolism and insulin resistance in patients with GDM. The serum levels of FABP4,FGF21 and Nesfatin-1 are significantly increased,and are related to insulin resistance.
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Objective To explore the predictive value of heart-type fatty acid-binding protein (HFABP) in predicting the cardiovascular events after none-cardiac thoracic surgery in aged patients.Methods Sixty eight aged patients were admitted from February 2012 to August 2014 and accepted none-cardiac thoracic surgery.The patients'general information and the incidence of postoperational cardiovascular events were recorded.Serum H-FABP was measured.The receiver operating characteristic (ROC) curve was constructed and the predictive effect of serum H-FABP for prognosis was analyzed.Results Eighteen patients were suffered from postoperative cardiovascular events (PCE).The level of H-FABP after surgery in patients with PCE was higher than that with none-PCE (P ＜ 0.05).The serum concentration of H-FABP after surgery and NT-proBNP before surgery was the independent risk factors for the elderly patients undergoing non-cardiac thoracic surgery.The area under ROC of H-FABP was 0.840 (95％ CI:0.716 ～0.964,P ＜0.01).The sensitivity and specificity of predicting postoperational cardiovascular event with serum concentration of H-FABP ＞5.26μg/L was 77.8％ and 84.0％ (OR 15.944,95％ CI:4.239 ～59.978,P ＜0.01).Conclusions The level of H-FABP after surgery is an independent risk factor for the occurrence of PCE for the elderly patients undergoing non-cardiac thoracic surgery.It can predict the risk of PCE after non-cardiac thoracic surgery in aged patients.
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ObjectiveTo determine the optimal timing of antenatal taurine supplementation to improve neuron and neural stem cell proliferation in fetal rats with intrauterine growth restriction.Methods Twenty-five pregnant SD rats were randomly divided into five groups (five rats in each group): group A was the control group, group B to E were the fetal growth restriction (FGR) model groups with low-protein diet during the experiment, group C, D, and E were supplemented with taurine [300 mg/(kg·d)] at day 9, 11 and 15, respectively. The birth weight of newborn rats was measured after natural delivery. The rats with body weight two standard deviations lower than the average weight in group A were diagnosed as FGR. There were five litters of newborn rats in each group, and two were randomly selected from each litter, resulting in ten newborn rats in each group. Proliferating cell nuclear antigen (PCNA) and fatty acid binding protein 7 (FABP7) positive cell expression in newborn rat brain tissues were detected by immunohistochemistry. Single factor analysis of variance, LSD tests were used for statistical analysis.ResultsThe average birth weight of newborn rats in group A, B, C, D and E were (6.61±0.45), (4.65±0.23), (5.37±0.17), (5.74±0.21), and (5.00±0.24) g, respectively. Average birth weight was lower in group B than in group A (t=2.447), higher in group D and E than in group B (t=2.306 and 2.306), higher in group D than in group C and E (t=2.306 and 2.306), and the differences were statistically significant (allP0.05). The IOD in group D was higher than that in group E, and the difference was statistically significant (t=4.182,P<0.05).ConclusionsAntenatal taurine supplementation can promote neuron and neural stem cell proliferation in rats with FGR. The effect is most obvious on the 11th day of pregnancy, and may lead to the promotion of brain development.
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Objective To observe the protective effect of ulinastatin (UTI) on the intestinal barrier function of septic rats.Methods Septic rat model was established using Sprague-Dawley rats by cecal ligation and puncture (CLP) method.Thirty Sprague-Dawley rats were randomly divided into 3 groups (n =10 for each grop) : sham group, septic group and UTI group.All rats received intraperitoneal injections of 0.9％ saline (10 mL/kg) after and 8 h after surgery.In UTI group, UTI (10 × 104 U/kg in 10 mL/kg saline) was injected after and 8 h after surgery.Collect blood samples after 0, 8, 12 h after surgery to examine levels of procalcitonin (PCT), intestinal fatty acid binding protein (iFABP) and diamine oxidase (DAO) by enzyme-linked immunosorbent assay (ELISA) method.Rats were killed 12 h after surgery to collect intestine tissue samples.Pathological changes of intestine were observed under microscopy, and the expression of tight junction protein-1 (ZO-1) and occludin were analyzed by Western blot.Results In sham group, the mucosa structure was complete and the shape was normal, and villi stood neatly.In septic group, intestinal was expanded, intertinal mucosal was atrophic, villi were scanty.An inflammatory infiltrate with numerous nuetrophils was found in the mucosal.In UTI group, the level of severity was relatively slight.The relative optical density of Western blot images were decreased on ZO-1 and occludin in CLP and UTI groups, and decreased more in CLP group (F =43.15 and 52.23, P ＜ 0.05).At 0h after surgery, the plasma values of PCT, iFABP and DAO were similar in three groups (F =11.17, 22.45 and 13.58, P ＞ 0.05).At 8h and 12h after surgery, values of PCT, iFABP and DAO in septic and UTI groups were much higher than those in the sham group, and those in UTI group were also significantly higher than those in septic group (F8h=85.26, 44.59 and 101.47, F12h =59.44, 49.26 and 69.57, all P＜0.05).PCT, iFABP and DAO levels were first increased and then fell down in sham group, those in septic group were keeping increasing, and those in UTI group were first increased and then kept stable.Conclusion UTI shows protective effect to intestinal barrier function in rats with sepsis.